Journal: Acta Pharmaceutica Sinica. B
Article Title: A critical role for Phocaeicola vulgatus in negatively impacting metformin response in diabetes
doi: 10.1016/j.apsb.2025.02.008
Figure Lengend Snippet: P. vulgatus -induced metformin nonresponse involved intestinal FXR signaling, but not exclusively. (A) Relative mRNA abundance of Fxr , its target genes, and Tgr5 mRNAs in the liver and ileum ( n = 5). (B) Western blot analysis of ileal FXR (i) and hepatic CYP7B1 (ii) protein expression and the statistical graph ( n = 3). (C, D) OGTT and HOMA-IR of P. vulgatus -colonized mice treated with GUDCA or TUDCA + CAPE ( n = 5–6). (E) Western blot analysis of ileal FXR (i) and hepatic CYP7B1 (ii) protein expression and the statistical graph ( n = 3). (F) Relative mRNA abundance of Fxr , its target genes, and Tgr5 mRNAs in the liver and ileum ( n = 5). (G, H) OGTT and ITT of E. coli -colonized mice treated with metformin or vehicle control ( n = 5). P value determined by two-tailed unpaired Student’s t -test, Mann–Whitney U test, Kruskal–Wallis test and one-way ANOVA. ns, non-significant. All data are presented as mean ± SEM or mean ± SD.
Article Snippet: The membrane was incubated with primary antibodies against FXR (Cell Signaling Technology, Cat# 72105, 1:1000), CYP7B1 (Proteintech, Cat# 24889-1-AP, 1:600), CYP7A1 (Abcam, Cat# ab65596, 1:1000), SHP (Absin, Cat# abs115798, 1:1000) or GAPDH (Cell Signaling Technology, Cat# 2118, 1:20,000) overnight at 4 °C.
Techniques: Western Blot, Expressing, Control, Two Tailed Test, MANN-WHITNEY