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fxr primary antibody  (Bioss)


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    Structured Review

    Bioss fxr primary antibody
    Fxr Primary Antibody, supplied by Bioss, used in various techniques. Bioz Stars score: 94/100, based on 30 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fxr primary antibody/product/Bioss
    Average 94 stars, based on 30 article reviews
    fxr primary antibody - by Bioz Stars, 2026-02
    94/100 stars

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    P. vulgatus -induced metformin nonresponse involved intestinal FXR signaling, but not exclusively. (A) Relative mRNA abundance of Fxr , its target genes, and Tgr5 mRNAs in the liver and ileum ( n = 5). (B) Western blot analysis of ileal FXR (i) and hepatic CYP7B1 (ii) protein expression and the statistical graph ( n = 3). (C, D) OGTT and HOMA-IR of P. vulgatus -colonized mice treated with GUDCA or TUDCA + CAPE ( n = 5–6). (E) Western blot analysis of ileal FXR (i) and hepatic CYP7B1 (ii) protein expression and the statistical graph ( n = 3). (F) Relative mRNA abundance of Fxr , its target genes, and Tgr5 mRNAs in the liver and ileum ( n = 5). (G, H) OGTT and ITT of E. coli -colonized mice treated with metformin or vehicle control ( n = 5). P value determined by two-tailed unpaired Student’s t -test, Mann–Whitney U test, Kruskal–Wallis test and one-way ANOVA. ns, non-significant. All data are presented as mean ± SEM or mean ± SD.

    Journal: Acta Pharmaceutica Sinica. B

    Article Title: A critical role for Phocaeicola vulgatus in negatively impacting metformin response in diabetes

    doi: 10.1016/j.apsb.2025.02.008

    Figure Lengend Snippet: P. vulgatus -induced metformin nonresponse involved intestinal FXR signaling, but not exclusively. (A) Relative mRNA abundance of Fxr , its target genes, and Tgr5 mRNAs in the liver and ileum ( n = 5). (B) Western blot analysis of ileal FXR (i) and hepatic CYP7B1 (ii) protein expression and the statistical graph ( n = 3). (C, D) OGTT and HOMA-IR of P. vulgatus -colonized mice treated with GUDCA or TUDCA + CAPE ( n = 5–6). (E) Western blot analysis of ileal FXR (i) and hepatic CYP7B1 (ii) protein expression and the statistical graph ( n = 3). (F) Relative mRNA abundance of Fxr , its target genes, and Tgr5 mRNAs in the liver and ileum ( n = 5). (G, H) OGTT and ITT of E. coli -colonized mice treated with metformin or vehicle control ( n = 5). P value determined by two-tailed unpaired Student’s t -test, Mann–Whitney U test, Kruskal–Wallis test and one-way ANOVA. ns, non-significant. All data are presented as mean ± SEM or mean ± SD.

    Article Snippet: The membrane was incubated with primary antibodies against FXR (Cell Signaling Technology, Cat# 72105, 1:1000), CYP7B1 (Proteintech, Cat# 24889-1-AP, 1:600), CYP7A1 (Abcam, Cat# ab65596, 1:1000), SHP (Absin, Cat# abs115798, 1:1000) or GAPDH (Cell Signaling Technology, Cat# 2118, 1:20,000) overnight at 4 °C.

    Techniques: Western Blot, Expressing, Control, Two Tailed Test, MANN-WHITNEY